ExplorEnz: EC 3.5.1.100

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3.5.1.100

Accepted name:

(R)-amidase

Reaction:

(1) (R)-piperazine-2-carboxamide + H₂O = (R)-piperazine-2-carboxylate + NH₃
(2) β-alaninamide + H₂O = β-alanine + NH₃

Other name(s):

R-stereospecific amidase; R-amidase

Systematic name:

(R)-piperazine-2-carboxamide amidohydrolase

Comments:

In addition (R)-piperidine-3-carboxamide is hydrolysed to (R)-piperidine-3-carboxylic acid and NH₃, and (R)-N-tert-butylpiperazine-2-carboxamide is hydrolysed to (R)-piperazine-2-carboxylic acid and tert-butylamine with lower activity. The enzyme does not act on the other amide substrates which are hydrolysed by EC 3.5.1.4 (amidase).

Links to other databases:

BRENDA, EXPASY, KEGG, MetaCyc

References:

1.	Komeda, H., Harada, H., Washika, S., Sakamoto, T., Ueda, M. and Asano, Y. A novel R-stereoselective amidase from Pseudomonas sp. MCI3434 acting on piperazine-2-tert-butylcarboxamide. Eur. J. Biochem. 271 (2004) 1580–1590. [DOI] [PMID: 15066183]

[EC 3.5.1.100 created 2009, modified 2011]