ExplorEnz: EC 3.4.24.38

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3.4.24.38

Accepted name:

gametolysin

Reaction:

Cleavage of the proline- and hydroxyproline-rich proteins of the Chlamydomonas cell wall; also cleaves azocasein, gelatin and Leu-Trp-Met┼Arg-Phe-Ala

Other name(s):

autolysin; Chlamydomonas cell wall degrading protease; lysin; Chlamydomonas reinhardtii metalloproteinase; gamete lytic enzyme; gamete autolysin

Comments:

A glycoprotein found in the periplasmic space of Chlamydomonas reinhardtii gametes in a 62 kDa inactive form; decreased to 60 kDa upon activation. A zinc enzyme, inhibited by phosphoramidon, but also thiol activated. Type example of peptidase family M11

Links to other databases:

BRENDA, EXPASY, KEGG, MetaCyc, MEROPS, CAS registry number: 97089-74-2

References:

1.	Jaenicke, L., Kunhe, W., Spessert, R., Wahle, U. and Waffenschmidt, S. Cell-wall lytic enzymes (autolysins) of Chlamydomonas reinhardtii are (hydroxy)proline-specific proteases. Eur. J. Biochem. 170 (1987) 485–491. [PMID: 3319620]
2.	Buchanan, M.J., Imam, S.H., Eskue, W.A. and Snell, W.J. Activation of the cell wall degrading protease, lysin, during sexual signalling in Chlamydomonas: the enzyme is stored as an inactive, higher relative molecular mass precursor in the periplasm. J. Cell. Biol. 108 (1989) 199–207. [PMID: 2910877]
3.	Matsuda, Y. Gametolysin. In: Barrett, A.J., Rawlings, N.D. & Woessner, J.F. (Ed.), Handbook of Proteolytic Enzymes, Academic Press, London, 1998, pp. 1140–1143.

[EC 3.4.24.38 created 1992, modified 2000]