ExplorEnz: EC 3.4.22.25

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3.4.22.25

Accepted name:

glycyl endopeptidase

Reaction:

Preferential cleavage: Gly┼, in proteins and small molecule substrates

Other name(s):

papaya peptidase B; papaya proteinase IV; glycine-specific proteinase; chymopapain; Papaya proteinase 4; PPIV; chymopapain M

Comments:

From the papaya plant, Carica papaya. Not inhibited by chicken cystatin, unlike most other homologues of papain, but in peptidase family C1 (papain family)

Links to other databases:

BRENDA, EXPASY, KEGG, MetaCyc, MEROPS, PDB, CAS registry number: 149719-24-4

References:

1.	Polgár, L. Isolation of highly active papaya peptidases A and B from commercial chymopapain. Biochim. Biophys. Acta 658 (1981) 262–269. [DOI] [PMID: 7018581]
2.	Buttle, D.J., Kembhavi, A.A., Sharp, S.L., Shute, R.E., Rich, D.H. and Barrett, A.J. Affinity purification of the novel cysteine proteinase papaya proteinase IV and papain from papaya latex. Biochem. J. 261 (1989) 469–476. [PMID: 2505761]
3.	Ritonja, A., Buttle, D.J., Rawlings, N.D., Turk, V. and Barrett, A.J. Papaya proteinase IV amino acid sequence. FEBS Lett. 258 (1989) 109–112. [DOI] [PMID: 2591528]
4.	Buttle, D.J., Ritonja, A., Pearl, L.H., Turk, V. and Barrett, A.J. Selective cleavage of glycyl bonds by papaya proteinase IV. FEBS Lett. 260 (1990) 195–197. [DOI] [PMID: 2404797]
5.	Buttle, D.J., Ritonja, A., Dando, P.M., Abrahamson, M., Shaw, E.N., Wikstrom, P., Turk, V. and Barrett, A.J. Interaction of papaya proteinase IV with inhibitors. FEBS Lett. 262 (1990) 58–60. [DOI] [PMID: 1690669]

[EC 3.4.22.25 created 1992]