| EC |
3.2.1.62 |
| Accepted name: |
glycosylceramidase |
| Reaction: |
a glycosyl-N-acylsphingosine + H2O = a ceramide + a sugar |
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For diagram of glycolipid biosynthesis, click here |
| Glossary: |
a ceramide = an N-acylsphingosine |
| Other name(s): |
phlorizin hydrolase; phloretin-glucosidase; glycosyl ceramide glycosylhydrolase; cerebrosidase; phloridzin β-glucosidase; lactase-phlorizin hydrolase; phloridzin glucosidase |
| Systematic name: |
glycosyl-N-acylsphingosine glycohydrolase |
| Comments: |
Broad specificity [cf. EC 3.2.1.45 (glucosylceramidase) and EC 3.2.1.46 (galactosylceramidase)]. Also hydrolyses phlorizin to phloretin and glucose. The intestinal enzyme is a complex that also catalyses the reaction of EC 3.2.1.108 lactase. |
| Links to other databases: |
BRENDA, EXPASY, KEGG, METACYC, CAS registry number: 9033-10-7 |
| References: |
| 1. |
Leese, H.J. and Semenza, G. On the identity between the small intestinal enzymes phlorizin hydrolase and glycosylceramidase. J. Biol. Chem. 248 (1973) 8170–8173. [PMID: 4752949] |
| 2. |
Lorenz-Meyer, H., Blum, A.L., Haemmerli, H.P. and Semenza, G. A second enzyme defect in acquired lactase deficiency: lack of small-intestinal phlorizin-hydrolase. Eur. J. Clin. Invest. 2 (1972) 326–331. [PMID: 5082068] |
| 3. |
Malathi, P. and Crane, R.K. Phlorizin hydrolase: a β-glucosidase of hamster intestinal brush border membrane. Biochim. Biophys. Acta 173 (1969) 245–256. [PMID: 5774775] |
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| [EC 3.2.1.62 created 1972, modified 1976] |
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