ExplorEnz: EC 3.2.1.197

Your query returned 1 entry. Printable version

3.2.1.197

Accepted name:

β-1,2-mannosidase

Reaction:

β-D-mannopyranosyl-(1→2)-β-D-mannopyranosyl-(1→2)-D-mannopyranose + H₂O = β-D-mannopyranosyl-(1→2)-D-mannopyranose + α-D-mannopyranose

Systematic name:

β-1,2-D-mannoside mannohydrolase

Comments:

The enzyme, characterized from multiple bacterial species, catalyses the hydrolysis of terminal, non-reducing D-mannose residues from β-1,2-mannotriose and β-1,2-mannobiose. The mechanism involves anomeric inversion, resulting in the release of α-D-mannopyranose. Activity with β-1,2-mannotriose or higher oligosaccharides is higher than that with β-1,2-mannobiose.

Links to other databases:

BRENDA, EXPASY, KEGG, MetaCyc

References:

1.	Cuskin, F., Basle, A., Ladeveze, S., Day, A.M., Gilbert, H.J., Davies, G.J., Potocki-Veronese, G. and Lowe, E.C. The GH130 family of mannoside phosphorylases contains glycoside hydrolases that target β-1,2-mannosidic linkages in Candida mannan. J. Biol. Chem. 290 (2015) 25023–25033. [DOI] [PMID: 26286752]
2.	Nihira, T., Chiku, K., Suzuki, E., Nishimoto, M., Fushinobu, S., Kitaoka, M., Ohtsubo, K. and Nakai, H. An inverting β-1,2-mannosidase belonging to glycoside hydrolase family 130 from Dyadobacter fermentans. FEBS Lett. 589 (2015) 3604–3610. [DOI] [PMID: 26476324]

[EC 3.2.1.197 created 2016]