The Enzyme Database

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EC 3.2.1.180     
Accepted name: unsaturated chondroitin disaccharide hydrolase
Reaction: β-D-4-deoxy-Δ4-GlcAp-(1→3)-β-D-GalNAc6S + H2O = 5-dehydro-4-deoxy-D-glucuronate + N-acetyl-β-D-galactosamine-6-O-sulfate
Glossary: 5-dehydro-4-deoxy-D-glucuronate = (4S,5R)-4,5-dihydroxy-2,6-dioxohexanoate
Other name(s): UGL (ambiguous); unsaturated glucuronyl hydrolase (ambiguous)
Systematic name: β-D-4-deoxy-Δ4-GlcAp-(1→3)-β-D-GalNAc6S hydrolase
Comments: The enzyme releases 4-deoxy-4,5-didehydro D-glucuronic acid or 4-deoxy-4,5-didehydro L-iduronic acid from chondroitin disaccharides, hyaluronan disaccharides and heparin disaccharides and cleaves both glycosidic (1→3) and (1→4) bonds. It prefers the sulfated disaccharides to the unsulfated disaccharides.
Links to other databases: BRENDA, EXPASY, KEGG, MetaCyc
References:
1.  Maruyama, Y., Nakamichi, Y., Itoh, T., Mikami, B., Hashimoto, W. and Murata, K. Substrate specificity of streptococcal unsaturated glucuronyl hydrolases for sulfated glycosaminoglycan. J. Biol. Chem. 284 (2009) 18059–18069. [PMID: 19416976]
2.  Nakamichi, Y., Maruyama, Y., Mikami, B., Hashimoto, W. and Murata, K. Structural determinants in streptococcal unsaturated glucuronyl hydrolase for recognition of glycosaminoglycan sulfate groups. J. Biol. Chem. 286 (2011) 6262–6271. [PMID: 21147778]
[EC 3.2.1.180 created 2011]
 
 


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