The enzyme from the tropical tree Dalbergia nigrescens Kurz belongs in glycosyl hydrolase family 1. The enzyme removes disaccharides from the natural substrates dalpatein 7-O-β-D-apiofuranosyl-(1→6)-β-D-glucopyranoside and 7-hydroxy-2′,4′,5′,6-tetramethoxy-7-O-β-D-apiofuranosyl-(1→6)-β-D-glucopyranoside (dalnigrein 7-O-β-D-apiofuranosyl-(1→6)-β-D-glucopyranoside) although it can also remove a single glucose residue from isoflavonoid 7-O-glucosides . Daidzin and genistin are also substrates.
Hosel, W. and Barz, W. β-Glucosidases from Cicer arietinum L. Purification and Properties of
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β-glycosidase and its substrates from Dalbergia nigrescens Kurz. Phytochemistry66 (2005) 1880–1889. [PMID: 16098548]
Ahn, Y.O., Mizutani, M., Saino, H. and Sakata, K. Furcatin hydrolase from Viburnum furcatum Blume is a novel
disaccharide-specific acuminosidase in glycosyl hydrolase family 1. J. Biol. Chem.279 (2004) 23405–23414. [PMID: 14976214]