The enzyme, isolated from the yeast Saccharomyces cerevisiae, is highly specific for inosine 5′-phosphate, and has no detectable activity with other purine and pyrimidine nucleotides. Requires divalent metals, such as Mg2+, Co2+ or Mn2+.
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Itoh, R., Saint-Marc, C., Chaignepain, S., Katahira, R., Schmitter, J.M. and Daignan-Fornier, B. The yeast ISN1 (YOR155c) gene encodes a new type of IMP-specific 5′-nucleotidase. BMC Biochem.4:4 (2003). [PMID: 12735798]