This is an endoribonuclease that cleaves double-stranded RNA molecules . The cleavage can be either a single-stranded nick or double-stranded break in the RNA, depending in part upon the degree of base-pairing in the region of the cleavage site . Specificity is conferred by negative determinants, i.e., the presence of certain Watson-Crick base-pairs at specific positions that strongly inhibit cleavage . RNase III is involved in both rRNA processing and mRNA processing and decay.
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