Exonucleolytic cleavage that removes extra residues from the 3′-terminus of tRNA to produce 5′-mononucleotides
Requires divalent cations for activity (Mg2+, Mn2+ or Co2+). Alteration of the 3′-terminal base has no effect on the rate of hydrolysis whereas modification of the 3′-terminal sugar has a major effect. tRNA terminating with a 3′-phosphate is completely inactive . This enzyme can convert a tRNA precursor into a mature tRNA .
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