The Enzyme Database

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EC 3.1.12.2     
Accepted name: DNA-3′-diphospho-5′-guanosine diphosphatase
Reaction: [DNA]-3′-diphospho-5′-guanosine + H2O = [DNA]-3′-phosphate + GMP
Other name(s): aprataxin; DNA-3′pp5′G guanylate hydrolase; APTX (gene name); HNT3 (gene name)
Systematic name: [DNA]-3′-diphospho-5′-guanosine hydrolase (guanosine 5′-phosphate-forming)
Comments: Aprataxin is a DNA-binding protein that catalyses (among other activities) the 3′ decapping of DNA-ppG (formed by EC 6.5.1.8, 3′-phosphate/5′-hydroxy nucleic acid ligase) [1]. The enzyme binds the guanylate group to a histidine residue at its active site, forming a covalent enzyme-nucleotide phosphate intermediate, followed by the hydrolysis of the guanylate from the nucleic acid and its eventual release. The enzyme also possesses the activity of EC 3.1.11.7, adenosine-5′-diphospho-5′-[DNA] diphosphatase, and EC 3.1.11.8, guanosine-5′-diphospho-5′-[DNA] diphosphatase.
Links to other databases: BRENDA, EXPASY, KEGG, MetaCyc
References:
1.  Das, U., Chauleau, M., Ordonez, H. and Shuman, S. Impact of DNA3′pp5′G capping on repair reactions at DNA 3′ ends. Proc. Natl. Acad. Sci. USA 111 (2014) 11317–11322. [PMID: 25049385]
2.  Chauleau, M., Jacewicz, A. and Shuman, S. DNA3′pp5′G de-capping activity of aprataxin: effect of cap nucleoside analogs and structural basis for guanosine recognition. Nucleic Acids Res. 43 (2015) 6075–6083. [PMID: 26007660]
[EC 3.1.12.2 created 2017]
 
 


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