ExplorEnz: EC 3.1.12.1

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3.1.12.1

Accepted name:

5′ to 3′ exodeoxyribonuclease (nucleoside 3′-phosphate-forming)

Reaction:

exonucleolytic cleavage in the 5′- to 3′-direction to yield nucleoside 3′-phosphates

Other name(s):

Cas4; 5′ to 3′ single stranded DNA exonuclease

Comments:

Preference for single-stranded DNA. The enzyme from the archaeon Sulfolobus solfataricus contains a [4Fe-4S] cluster and requires a divalent metal cation, such as Mg²⁺ or Mn²⁺, for activity.

Links to other databases:

BRENDA, EXPASY, KEGG, MetaCyc, PDB

References:

1.	Zhang, J., Kasciukovic, T. and White, M.F. The CRISPR associated protein Cas4 Is a 5′ to 3′ DNA exonuclease with an iron-sulfur cluster. PLoS One 7:e47232 (2012). [DOI] [PMID: 23056615]
2.	Lemak, S., Beloglazova, N., Nocek, B., Skarina, T., Flick, R., Brown, G., Popovic, A., Joachimiak, A., Savchenko, A. and Yakunin, A.F. Toroidal structure and DNA cleavage by the CRISPR-associated [4Fe-4S] cluster containing Cas4 nuclease SSO0001 from Sulfolobus solfataricus. J. Am. Chem. Soc. 135 (2013) 17476–17487. [DOI] [PMID: 24171432]

[EC 3.1.12.1 created 2014]