Brings about the final exonucleolytic trimming of the 3′-terminus of tRNA precursors in Escherichia coli by a phosphorolysis, producing a mature 3′-terminus on tRNA and nucleoside diphosphate. Not identical with EC 22.214.171.124 polyribonucleotide nucleotidyltransferase.
Cudny, H. and Deutscher, M.P. 3′ processing of tRNA precursors in ribonuclease-deficient Escherichia coli. Development and characterization of an in vitro processing system and evidence for a phosphate requirement. J. Biol. Chem.263 (1988) 1518–1523. [PMID: 3275667]
Deutscher, M.P., Marshall, G.T. and Cudny, H. RNase PH: an Escherichia coli phosphate-dependent nuclease distinct from polynucleotide phosphorylase. Proc. Natl. Acad. Sci. USA85 (1988) 4710–4714. [DOI] [PMID: 2455297]