This enzyme is strictly specific for UMP as substrate and is used by prokaryotes in the de novo synthesis of pyrimidines, in contrast to eukaryotes, which use the dual-specificity enzyme UMP/CMP kinase (EC 184.108.40.206) for the same purpose . This enzyme is the subject of feedback regulation, being inhibited by UTP and activated by GTP .
Serina, L., Blondin, C., Krin, E., Sismeiro, O., Danchin, A., Sakamoto, H., Gilles, A.M. and Bârzu, O. Escherichia coli UMP-kinase, a member of the aspartokinase family, is a
hexamer regulated by guanine nucleotides and UTP. Biochemistry34 (1995) 5066–5074. [PMID: 7711027]
Marco-Marín, C., Gil-Ortiz, F. and Rubio, V. The crystal structure of Pyrococcus furiosus UMP kinase provides insight
into catalysis and regulation in microbial pyrimidine nucleotide
biosynthesis. J. Mol. Biol.352 (2005) 438–454. [DOI] [PMID: 16095620]