ExplorEnz: EC 2.7.1.160

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2.7.1.160

Accepted name:

2′-phosphotransferase

Reaction:

2′-phospho-[ligated tRNA] + NAD⁺ = mature tRNA + ADP-ribose 1′′,2′′-phosphate + nicotinamide

For diagram of tRNA splicing, click here

Glossary:

ADP-ribose = adenosine 5′-(5-deoxy-D-ribofuranos-5-yl diphosphate)

Other name(s):

yeast 2′-phosphotransferase; Tpt1; Tpt1p; 2′-phospho-tRNA:NAD⁺ phosphotransferase

Systematic name:

2′-phospho-[ligated tRNA]:NAD⁺ phosphotransferase

Comments:

Catalyses the final step of tRNA splicing in the yeast Saccharomyces cerevisiae [2]. The reaction takes place in two steps: in the first step, the 2′-phosphate on the RNA substrate is ADP-ribosylated, causing the relase of nicotinamide and the formation of the reaction intermediate, ADP-ribosylated tRNA [6]. In the second step, dephosphorylated (mature) tRNA is formed along with ADP ribose 1′′-2′′-cyclic phosphate. Highly specific for oligonucleotide substrates bearing an internal 2′-phosphate. Oligonucleotides with only a terminal 5′- or 3′-phosphate are not substrates [1].

Links to other databases:

BRENDA, EXPASY, KEGG, MetaCyc, PDB, CAS registry number: 126905-00-8

References:

1.	Steiger, M.A., Kierzek, R., Turner, D.H. and Phizicky, E.M. Substrate recognition by a yeast 2′-phosphotransferase involved in tRNA splicing and by its Escherichia coli homolog. Biochemistry 40 (2001) 14098–14105. [DOI] [PMID: 11705403]
2.	Spinelli, S.L., Kierzek, R., Turner, D.H. and Phizicky, E.M. Transient ADP-ribosylation of a 2′-phosphate implicated in its removal from ligated tRNA during splicing in yeast. J. Biol. Chem. 274 (1999) 2637–2644. [DOI] [PMID: 9915792]
3.	Culver, G.M., McCraith, S.M., Consaul, S.A., Stanford, D.R. and Phizicky, E.M. A 2′-phosphotransferase implicated in tRNA splicing is essential in Saccharomyces cerevisiae. J. Biol. Chem. 272 (1997) 13203–13210. [DOI] [PMID: 9148937]
4.	McCraith, S.M. and Phizicky, E.M. An enzyme from Saccharomyces cerevisiae uses NAD⁺ to transfer the splice junction 2′-phosphate from ligated tRNA to an acceptor molecule. J. Biol. Chem. 266 (1991) 11986–11992. [PMID: 2050693]
5.	Hu, Q.D., Lu, H., Huo, K., Ying, K., Li, J., Xie, Y., Mao, Y. and Li, Y.Y. A human homolog of the yeast gene encoding tRNA 2′-phosphotransferase: cloning, characterization and complementation analysis. Cell. Mol. Life Sci. 60 (2003) 1725–1732. [DOI] [PMID: 14504659]
6.	Steiger, M.A., Jackman, J.E. and Phizicky, E.M. Analysis of 2′-phosphotransferase (Tpt1p) from Saccharomyces cerevisiae: evidence for a conserved two-step reaction mechanism. RNA 11 (2005) 99–106. [DOI] [PMID: 15611300]
7.	Sawaya, R., Schwer, B. and Shuman, S. Structure-function analysis of the yeast NAD⁺-dependent tRNA 2′-phosphotransferase Tpt1. RNA 11 (2005) 107–113. [DOI] [PMID: 15611301]
8.	Kato-Murayama, M., Bessho, Y., Shirouzu, M. and Yokoyama, S. Crystal structure of the RNA 2′-phosphotransferase from Aeropyrum pernix K1. J. Mol. Biol. 348 (2005) 295–305. [DOI] [PMID: 15811369]

[EC 2.7.1.160 created 2006]