Requires Mg2+. The enzyme is modified by binding of the first substrate, phytyl diphosphate, before reaction of the modified enzyme with the second substrate, chlorophyllide a, can occur. The reaction also occurs when phytyl diphosphate is replaced by geranylgeranyl diphosphate.
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Oster, U., Bauer, C.E. and Rüdiger, W. Characterization of chlorophyll a and bacteriochlorophyll a synthases by heterologous expression in Escherichia coli. J. Biol. Chem.272 (1997) 9671–9676. [PMID: 9092496]
Rüdiger, W., Benz, J. and Guthoff, C. Detection and partial characterization of activity of chlorophyll synthetase in etioplast membranes. Eur. J. Biochem.109 (1980) 193–200. [PMID: 7408876]