The Enzyme Database

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EC 2.3.1.215     
Accepted name: anthocyanidin 3-O-glucoside 6′′-O-acyltransferase
Reaction: 4-hydroxycinnamoyl-CoA + an anthocyanidin 3-O-β-D-glucoside = CoA + an anthocyanidin 3-O-[6-O-(4-hydroxycinnamoyl)-β-D-glucoside]
For diagram of anthocyanidin acylglucoside biosynthesis, click here and for diagram of salvianin biosynthesis, click here
Glossary: 4-hydroxycinnamoyl-CoA = 4-coumaroyl-CoA
3,4-dihydroxycinnamoyl-CoA = caffeoyl-CoA
cyanidin = 3,3′,4′,5,7-pentahydroxyflavylium
delphinidin = 3,3′,4′,5,5′,7-hexahydroxyflavylium
Systematic name: 4-hydroxycinnamoyl-CoA:anthocyanin-3-O-glucoside 6′′-O-acyltransferase
Comments: Isolated from the plants Perilla frutescens and Gentiana triflora (clustered gentian). Acts on a range of anthocyanidin 3-O-glucosides, 3,5-di-O-glucosides and cyanidin 3-rutinoside. It did not act on delphinidin 3,3′,7-tri-O-glucoside. Recombinant Perilla frutescens enzyme could utilize caffeoyl-CoA but not malonyl-CoA as alternative acyl donor.
Links to other databases: BRENDA, EXPASY, KEGG, MetaCyc
References:
1.  Fujiwara, H., Tanaka, Y., Fukui, Y., Ashikari, T., Yamaguchi, M. and Kusumi, T. Purification and characterization of anthocyanin 3-aromatic acyltransferase from Perilla frutescens. Plant Sci. 137 (1998) 87–94.
2.  Yonekura-Sakakibara, K., Tanaka, Y., Fukuchi-Mizutani, M., Fujiwara, H., Fukui, Y., Ashikari, T., Murakami, Y., Yamaguchi, M. and Kusumi, T. Molecular and biochemical characterization of a novel hydroxycinnamoyl-CoA: anthocyanin 3-O-glucoside-6"-O-acyltransferase from Perilla frutescens. Plant Cell Physiol 41 (2000) 495–502. [PMID: 10845463]
[EC 2.3.1.215 created 2013]
 
 


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