The enzyme was studied using the semisynthetic substrate 2-debenzoyl-7,13-diacetylbaccatin III. It will not acylate the hydroxy group at 1β, 7β, 10β or 13α of 10-deacetyl baccatin III, or at 2α or 5α of taxa-4(20),11-diene-2α,5α-diol.
Walker, K. and Croteau, R. Taxol biosynthesis: molecular cloning of a benzoyl-CoA:taxane 2α-O-benzoyltransferase cDNA from taxus and functional expression in Escherichia coli. Proc. Natl. Acad. Sci. USA97 (2000) 13591. [DOI] [PMID: 11095755]