ExplorEnz: EC 2.1.1.34

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2.1.1.34

Accepted name:

tRNA (guanosine¹⁸-2′-O)-methyltransferase

Reaction:

S-adenosyl-L-methionine + guanosine¹⁸ in tRNA = S-adenosyl-L-homocysteine + 2′-O-methylguanosine¹⁸ in tRNA

Other name(s):

tRNA (Gm18) 2′-O-methyltransferase; tRNA (Gm18) methyltransferase; TrmH; SpoU

Systematic name:

S-adenosyl-L-methionine:tRNA (guanosine¹⁸-2′-O)-methyltransferase

Comments:

The enzyme catalyses the methylation of guanosine¹⁸ in tRNA.

Links to other databases:

BRENDA, EXPASY, KEGG, MetaCyc, PDB, CAS registry number: 37257-01-5

References:

1.	Gefter, M.L. The in vitro synthesis of 2′-O-methylguanosine and 2-methylthio ⁶N (γ,gamma-dimethylallyl) adenosine in transfer RNA of Escherichia coli. Biochem. Biophys. Res. Commun. 36 (1969) 435–441. [DOI] [PMID: 4898378]
2.	Kumagai, I., Watanabe, K. and Oshima, T. Thermally induced biosynthesis of 2′-O-methylguanosine in tRNA from an extreme thermophile, Thermus thermophilus HB27. Proc. Natl. Acad. Sci. USA 77 (1980) 1922–1926. [DOI] [PMID: 6990416]
3.	Hori, H., Yamazaki, N., Matsumoto, T., Watanabe, Y., Ueda, T., Nishikawa, K., Kumagai, I. and Watanabe, K. Substrate recognition of tRNA (guanosine-2′-)-methyltransferase from Thermus thermophilus HB27. J. Biol. Chem. 273 (1998) 25721–25727. [DOI] [PMID: 9748240]
4.	Pleshe, E., Truesdell, J. and Batey, R.T. Structure of a class II TrmH tRNA-modifying enzyme from Aquifex aeolicus. Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. 61 (2005) 722–728. [DOI] [PMID: 16511140]
5.	Ochi, A., Makabe, K., Kuwajima, K. and Hori, H. Flexible recognition of the tRNA G18 methylation target site by TrmH methyltransferase through first binding and induced fit processes. J. Biol. Chem. 285 (2010) 9018–9029. [DOI] [PMID: 20053984]

[EC 2.1.1.34 created 1972, modified 2005, modified 2011]