ExplorEnz: EC 2.1.1.33

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2.1.1.33

Accepted name:

tRNA (guanine⁴⁶-N⁷)-methyltransferase

Reaction:

S-adenosyl-L-methionine + guanine⁴⁶ in tRNA = S-adenosyl-L-homocysteine + N⁷-methylguanine⁴⁶ in tRNA

Other name(s):

Trm8/Trm82; TrmB; tRNA (m⁷G⁴⁶) methyltransferase; transfer ribonucleate guanine 7-methyltransferase; 7-methylguanine transfer ribonucleate methylase; tRNA guanine 7-methyltransferase; N⁷-methylguanine methylase; S-adenosyl-L-methionine:tRNA (guanine-7-N-)-methyltransferase

Systematic name:

S-adenosyl-L-methionine:tRNA (guanine-N⁷)-methyltransferase

Comments:

The enzyme specifically methylates guanine⁴⁶ at N⁷ in tRNA.

Links to other databases:

BRENDA, EXPASY, KEGG, MetaCyc, PDB, CAS registry number: 37257-00-4

References:

1.	Aschhoff, H.J., Elten, H., Arnold, H.H., Mahal, G., Kersten, W. and Kersten, H. 7-Methylguanine specific tRNA-methyltransferase from Escherichia coli. Nucleic Acids Res. 3 (1976) 3109–3122. [DOI] [PMID: 794833]
2.	Zegers, I., Gigot, D., van Vliet, F., Tricot, C., Aymerich, S., Bujnicki, J.M., Kosinski, J. and Droogmans, L. Crystal structure of Bacillus subtilis TrmB, the tRNA (m⁷G⁴⁶) methyltransferase. Nucleic Acids Res. 34 (2006) 1925–1934. [DOI] [PMID: 16600901]
3.	Purta, E., van Vliet, F., Tricot, C., De Bie, L.G., Feder, M., Skowronek, K., Droogmans, L. and Bujnicki, J.M. Sequence-structure-function relationships of a tRNA (m⁷G⁴⁶) methyltransferase studied by homology modeling and site-directed mutagenesis. Proteins 59 (2005) 482–488. [DOI] [PMID: 15789416]
4.	Liu, Q., Gao, Y., Yang, W., Zhou, H., Gao, Y., Zhang, X., Teng, M. and Niu, L. Crystallization and preliminary crystallographic analysis of tRNA (m⁷G46) methyltransferase from Escherichia coli. Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. 64 (2008) 743–745. [DOI] [PMID: 18678947]
5.	Alexandrov, A., Martzen, M.R. and Phizicky, E.M. Two proteins that form a complex are required for 7-methylguanosine modification of yeast tRNA. RNA 8 (2002) 1253–1266. [PMID: 12403464]

[EC 2.1.1.33 created 1972, modified 2011]