ExplorEnz: EC 2.1.1.190

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2.1.1.190

Accepted name:

23S rRNA (uracil¹⁹³⁹-C⁵)-methyltransferase

Reaction:

S-adenosyl-L-methionine + uracil¹⁹³⁹ in 23S rRNA = S-adenosyl-L-homocysteine + 5-methyluracil¹⁹³⁹ in 23S rRNA

Other name(s):

RumA; RNA uridine methyltransferase A; YgcA

Systematic name:

S-adenosyl-L-methionine:23S rRNA (uracil¹⁹³⁹-C⁵)-methyltransferase

Comments:

The enzyme specifically methylates uracil¹⁹³⁹ at C⁵ in 23S rRNA [1]. The enzyme contains an [4Fe-4S] cluster coordinated by four conserved cysteine residues [2].

Links to other databases:

BRENDA, EXPASY, KEGG, MetaCyc, PDB

References:

1.	Agarwalla, S., Kealey, J.T., Santi, D.V. and Stroud, R.M. Characterization of the 23 S ribosomal RNA m⁵U¹⁹³⁹ methyltransferase from Escherichia coli. J. Biol. Chem. 277 (2002) 8835–8840. [DOI] [PMID: 11779873]
2.	Lee, T.T., Agarwalla, S. and Stroud, R.M. Crystal structure of RumA, an iron-sulfur cluster containing E. coli ribosomal RNA 5-methyluridine methyltransferase. Structure 12 (2004) 397–407. [DOI] [PMID: 15016356]
3.	Madsen, C.T., Mengel-Jorgensen, J., Kirpekar, F. and Douthwaite, S. Identifying the methyltransferases for m⁵U⁷⁴⁷ and m⁵U¹⁹³⁹ in 23S rRNA using MALDI mass spectrometry. Nucleic Acids Res. 31 (2003) 4738–4746. [PMID: 12907714]
4.	Persaud, C., Lu, Y., Vila-Sanjurjo, A., Campbell, J.L., Finley, J. and O'Connor, M. Mutagenesis of the modified bases, m⁵U¹⁹³⁹ and Ψ²⁵⁰⁴, in Escherichia coli 23S rRNA. Biochem. Biophys. Res. Commun. 392 (2010) 223–227. [DOI] [PMID: 20067766]
5.	Agarwalla, S., Stroud, R.M. and Gaffney, B.J. Redox reactions of the iron-sulfur cluster in a ribosomal RNA methyltransferase, RumA: optical and EPR studies. J. Biol. Chem. 279 (2004) 34123–34129. [DOI] [PMID: 15181002]
6.	Lee, T.T., Agarwalla, S. and Stroud, R.M. A unique RNA Fold in the RumA-RNA-cofactor ternary complex contributes to substrate selectivity and enzymatic function. Cell 120 (2005) 599–611. [DOI] [PMID: 15766524]

[EC 2.1.1.190 created 2010]