The enzyme is found in sulfate-reducing bacteria. The source of the electrons is molecular hydrogen, via EC 220.127.116.11, cytochrome-c3 hydrogenase. The organisms utilize the sulfite that is produced for energy generation by EC 18.104.22.168, dissimilatory sulfite reductase.
Ishimoto, M. and Koyama, J. On the role of a cytochrome in the thiosulfate reduction by sulfate-reducing bacterium. B. Chem. Soc. Jpn.28 (1955) 231b–232.
Ishimoto, M., Toyama, J. Biochemical studies on sulfate reducing bacteria. VI. Separation of hydrogenase and thiosulfate reductase and partial purification of cytochrome and green pigment. J. Biochem. (Tokyo)44 (1957) 233–242.
Nakatsukasa, W. and Akagi, J.M. Thiosulfate reductase isolated from Desulfotomaculum nigrificans. J. Bacteriol.98 (1969) 429–433. [PMID: 5784203]
Haschke, R.H. and Campbell, L.L. Thiosulfate reductase of Desulfovibrio vulgaris. J. Bacteriol.106 (1971) 603–607. [PMID: 5573735]
Hatchikian, E.C. Purification and properties of thiosulfate reductase from Desulfovibrio gigas. Arch. Microbiol.105 (1975) 249–256. [PMID: 242299]
Aketagawa, J., Kobayashi, K. and Ishimoto, M. Purification and properties of thiosulfate reductase from Desulfovibrio vulgaris, Miyazaki F. J. Biochem.97 (1985) 1025–1032. [PMID: 2993256]