| EC |
1.7.3.1 |
| Accepted name: |
nitroalkane oxidase |
| Reaction: |
a nitroalkane + H2O + O2 = an aldehyde or ketone + nitrite + H2O2 |
| Other name(s): |
nitroethane oxidase; NAO; nitroethane:oxygen oxidoreductase |
| Systematic name: |
nitroalkane:oxygen oxidoreductase |
| Comments: |
Has an absolute requirement for FAD [4]. While nitroethane may be the physiological substrate [2], the enzyme also acts on several other nitroalkanes, including 1-nitropropane, 2-nitropropane, 1-nitrobutane, 1-nitropentane, 1-nitrohexane, nitrocyclohexane and some nitroalkanols [4]. Differs from EC 1.13.12.16, nitronate monooxygenase, in that the preferred substrates are neutral nitroalkanes rather than anionic nitronates [4]. |
| Links to other databases: |
BRENDA, EAWAG-BBD, EXPASY, KEGG, MetaCyc, PDB, CAS registry number: 9029-36-1, 65802-82-6 |
| References: |
| 1. |
Little, H.N. Oxidation of nitroethane by extracts from Neurospora. J. Biol. Chem. 193 (1951) 347–358. [PMID: 14907722] |
| 2. |
Kido, T., Hashizume, K. and Soda, K. Purification and properties of nitroalkane oxidase from Fusarium oxysporum. J. Bacteriol. 133 (1978) 53–58. [PMID: 22538] |
| 3. |
Daubner, S.C., Gadda, G., Valley, M.P. and Fitzpatrick, P.F. Cloning of nitroalkane oxidase from Fusarium oxysporum identifies a new
member of the acyl-CoA dehydrogenase superfamily. Proc. Natl. Acad. Sci. USA 99 (2002) 2702–2707. [DOI] [PMID: 11867731] |
| 4. |
Fitzpatrick, P.F., Orville, A.M., Nagpal, A. and Valley, M.P. Nitroalkane oxidase, a carbanion-forming flavoprotein homologous to
acyl-CoA dehydrogenase. Arch. Biochem. Biophys. 433 (2005) 157–165. [DOI] [PMID: 15581574] |
| 5. |
Valley, M.P., Tichy, S.E. and Fitzpatrick, P.F. Establishing the kinetic competency of the cationic imine intermediate in
nitroalkane oxidase. J. Am. Chem. Soc. 127 (2005) 2062–2066. [DOI] [PMID: 15713081] |
|
| [EC 1.7.3.1 created 1961, modified 2006, modified 2009] |
| |
|
| |
|
Printable version