EC	1.4.1.28
Accepted name:	secondary-alkyl amine dehydrogenase [NAD(P)+]
Reaction:	a secondary-alkyl amine + H2O + NAD(P)+ = a ketone + NH3 + NAD(P)H + H+
Glossary:	a secondary-alkyl amine = RCHNH2R′
Other name(s):	AmDH (ambiguous); amine dehydrogenase (ambiguous)
Systematic name:	secondary-alkyl amine:NAD(P)+ oxidoreductase (deaminating)
Comments:	The enzyme has been shown to react preferentially with short-chain ketones such as cyclohexanone, primary amine groups attached to secondary alkyl groups, or D- and L-amino acids. It also reduces aldehydes to primary amines. Cosubstrate preference depends on the substrate.
References:	1.  Itoh, N., Yachi, C. and Kudome, T. Determining a novel NAD+-dependent amine dehydrogenase with a broad substrate range from Streptomyces virginiae IFO 12827: purification and characterization. Journal of Molecular Catalysis B: Enzymatic 10 (2000) 281–290. 2.  Mayol, O., David, S., Darii, E., Debard, A., Mariage, A., Pellouin, V., Petit, J.L., Salanoubat, M., de Berardinis, V., Zaparucha, A. and Vergne-Vaxelaire, C. Asymmetric reductive amination by a wild-type amine dehydrogenase from the thermophilic bacteria Petrotoga mobilis. Catalysis Science & Technology 6 (2016) 7421–7428. 3.  Mayol, O., Bastard, K., Beloti, L., Frese, A., Turkenburg, J.P., Petit, J.L., Mariage, A., Debard, A., Pellouin, V., Perret, A., de Berardinis, V., Zaparucha, A., Grogan, G. and Vergne-Vaxelaire, C. A family of native amine dehydrogenases for the asymmetric reductive amination of ketones. Nature Catalysis 2 (2019) 324–333. 4.  Lee, S., Jeon, H., Giri, P., Lee, U.J., Jung, H., Lim, S., Sarak, S., Khobragade, T.P., Kim, B.G. and Yun, H. The reductive amination of carbonyl compounds using native amine dehydrogenase from Laribacter hongkongensis. Biotechnol. Bioprocess Eng. 26 (2021) 384–391.
[EC 1.4.1.28 created 2022]