| EC |
1.17.1.3 |
| Accepted name: |
leucoanthocyanidin reductase |
| Reaction: |
(2R,3S)-catechin + NADP+ + H2O = 2,3-trans-3,4-cis-leucocyanidin + NADPH + H+ |
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For diagram of flavonoid biosynthesis, click here |
| Other name(s): |
leucocyanidin reductase |
| Systematic name: |
(2R,3S)-catechin:NADP+ 4-oxidoreductase |
| Comments: |
The enzyme catalyses the synthesis of catechin, catechin-4β-ol (leucocyanidin) and the related flavan-3-ols afzelechin and gallocatechin, which are initiating monomers in the synthesis of plant polymeric proanthocyanidins or condensed tannins. While 2,3-trans-3,4-cis-leucocyanidin is the preferred flavan-3,4-diol substrate, 2,3-trans-3,4-cis-leucodelphinidin and 2,3-trans-3,4-cis-leucopelargonidin can also act as substrates, but more slowly. NADH can replace NADPH but is oxidized more slowly. |
| Links to other databases: |
BRENDA, EXPASY, KEGG, MetaCyc, PDB, CAS registry number: 776323-46-7 |
| References: |
| 1. |
Tanner, G.J. and Kristiansen, K.N. Synthesis of 3,4-cis-[3H]leucocyanidin and enzymatic reduction to catechin. Anal. Biochem. 209 (1993) 274–277. [DOI] [PMID: 8470799] |
| 2. |
Tanner, G.J., Francki, K.T., Abrahams, S., Watson, J.M., Larkin, P.J. and Ashton, A.R. Proanthocyanidin biosynthesis in plants: Purification of legume leucoanthocyanidin reductase and molecular cloning of its cDNA. J. Biol. Chem. 278 (2003) 31647–31656. [DOI] [PMID: 12788945] |
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| [EC 1.17.1.3 created 2003] |
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