The Enzyme Database

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EC 1.14.99.22     
Accepted name: ecdysone 20-monooxygenase
Reaction: ecdysone + AH2 + O2 = 20-hydroxyecdysone + A + H2O
Other name(s): α-ecdysone C-20 hydroxylase; ecdysone 20-hydroxylase
Systematic name: Ecdysone,hydrogen-donor:oxygen oxidoreductase (20-hydroxylating)
Comments: An enzyme from insect fat body or malpighian tubules involving a heme-thiolate protein (P-450). NADPH can act as ultimate hydrogen donor.
Links to other databases: BRENDA, EXPASY, KEGG, MetaCyc, CAS registry number: 55071-97-1
References:
1.  Johnson, P. and Rees, H.H. The mechanism of C-20 hydroxylation of α-ecdysone in the desert locust, Schistocerca gregaria. Biochem. J. 168 (1977) 513–520. [PMID: 606249]
2.  Nigg, H.N., Svoboda, J.A., Thompson, M.J., Dutky, S.R., Kaplanis, J.N. and Robbins, W.E. Ecdysome 20-hydroxylase from the midgut of the tobacco hornworm (Manduca sexta L.). Experientia 32 (1976) 438–439. [PMID: 5286]
3.  Smith, S.L., Bollenbacher, W.E., Cooper, D.Y., Schleyer, H., Wielgus, J.J. and Gilbert, L.I. Ecdysone 20-monooxygenase: characterization of an insect cytochrome p-450 dependent steroid hydroxylase. Mol. Cell. Endocrinol. 15 (1979) 111–133. [PMID: 488526]
[EC 1.14.99.22 created 1978]
 
 


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