ExplorEnz: EC 1.14.19.34

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1.14.19.34

Accepted name:

acyl-lipid (9+3)-(E)-desaturase

Reaction:

(1) an oleoyl-[glycerolipid] + 2 ferrocytochrome b₅ + O₂ + 2 H⁺ = a (9Z,12E)-octadeca-9,12-dienoyl-[glycerolipid] + 2 ferricytochrome b₅ + 2 H₂O
(2) a palmitoleoyl-[glycerolipid] + 2 ferrocytochrome b₅ + O₂ + 2 H⁺ = a (9Z,12E)-hexadeca-9,12-dienoyl-[glycerolipid] + 2 ferricytochrome b₅ + 2 H₂O

Other name(s):

acyl-lipid 12-(E)-desaturase; DsFAD2-1; FADX

Systematic name:

Δ⁹ acyl-lipid,ferrocytochrome b₅:oxygen oxidoreductase (12,13 trans-dehydrogenating)

Comments:

The enzymes from the plants Dimorphotheca sinuata (African daisy) and Vernicia fordii (tung oil tree) insert a trans double bond in position C-12 of oleate and palmitoleate incorporated into glycerolipids. The enzyme introduces the new double bond at a position three carbons away from an existing double bond at position 9, towards the methyl end of the fatty acid. The enzyme from tung oil tree also possesses the activity of EC 1.14.19.33, Δ¹² acyl-lipid conjugase.

Links to other databases:

BRENDA, EXPASY, KEGG, MetaCyc

References:

1.	Dyer, J.M., Chapital, D.C., Kuan, J.C., Mullen, R.T., Turner, C., McKeon, T.A. and Pepperman, A.B. Molecular analysis of a bifunctional fatty acid conjugase/desaturase from tung. Implications for the evolution of plant fatty acid diversity. Plant Physiol. 130 (2002) 2027–2038. [DOI] [PMID: 12481086]
2.	Cahoon, E.B. and Kinney, A.J. Dimorphecolic acid is synthesized by the coordinate activities of two divergent Δ¹²-oleic acid desaturases. J. Biol. Chem. 279 (2004) 12495–12502. [DOI] [PMID: 14718523]

[EC 1.14.19.34 created 2015]