ExplorEnz: EC 1.14.15.19

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1.14.15.19

Accepted name:

C-19 steroid 1α-hydroxylase

Reaction:

testosterone + 2 reduced ferredoxin [iron-sulfur] cluster + O₂ + 2 H⁺ = 1α-hydroxytestosterone + H₂O + 2 oxidized ferredoxin [iron-sulfur] cluster

Other name(s):

CYP260A1

Systematic name:

testosterone,reduced-ferredoxin:oxygen oxidoreductase (1α-hydroxylating)

Comments:

The enzyme, characterized from the bacterium Sorangium cellulosum, is a class I cytochrome P-450, and uses ferredoxin as its electron donor [1]. It was shown to act on several C-19 steroid substrates, including testosterone, androstenedione, testosterone-acetate and 11-oxoandrostenedione [2].

Links to other databases:

BRENDA, EXPASY, KEGG, MetaCyc

References:

1.	Ewen, K.M., Hannemann, F., Khatri, Y., Perlova, O., Kappl, R., Krug, D., Huttermann, J., Muller, R. and Bernhardt, R. Genome mining in Sorangium cellulosum So ce56: identification and characterization of the homologous electron transfer proteins of a myxobacterial cytochrome P450. J. Biol. Chem. 284 (2009) 28590–28598. [DOI] [PMID: 19696019]
2.	Khatri, Y., Ringle, M., Lisurek, M., von Kries, J.P., Zapp, J. and Bernhardt, R. Substrate hunting for the myxobacterial CYP260A1 revealed new 1α-hydroxylated products from C-19 steroids. ChemBioChem 17 (2016) 90–101. [DOI] [PMID: 26478560]

[EC 1.14.15.19 created 2016]