The Enzyme Database

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EC 1.14.15.18     
Accepted name: calcidiol 1-monooxygenase
Reaction: (1) calcidiol + 2 reduced adrenodoxin + 2 H+ + O2 = calcitriol + 2 oxidized adrenodoxin + H2O
(2) secalciferol + 2 reduced adrenodoxin + 2 H+ + O2 = calcitetrol + 2 oxidized adrenodoxin + H2O
For diagram of calciferol biosynthesis, click here
Glossary: calcidiol = 25-hydroxyvitamin D3 = (3S,5Z,7E)-9,10-seco-5,7,10(19)-cholestatriene-3,25-diol
calcitriol = 1α,25-dihydroxyvitamin D3 = (1S,3R,5Z,7E)-9,10-seco-5,7,10(19)-cholestatriene-1,3,25-triol
secalciferol = (24R),25-dihydroxyvitamin D3 = (3S,5Z,7E,24R)-9,10-secocholesta-5,7,10-triene-3,24,25-triol
calcitetrol = 1α,(24R),25-dihydroxyvitamin D3 = (1S,3R,5Z,7E,24R,25)-9,10-secocholesta-5,7,10(19)-triene-1,3,24,25-tetrol
Other name(s): 25-hydroxycholecalciferol 1-hydroxylase; 25-hydroxycholecalciferol 1-monooxygenase; 1-hydroxylase-25-hydroxyvitamin D3; 25-hydroxy D3-1α-hydroxylase; 25-hydroxycholecalciferol 1α-hydroxylase; 25-hydroxyvitamin D3 1α-hydroxylase
Systematic name: calcidiol,adrenodoxin:oxygen oxidoreductase (1-hydroxylating)
Comments: A P-450 (heme-thiolate) enzyme found in mammals.
Links to other databases: BRENDA, EXPASY, KEGG, MetaCyc, CAS registry number: 9081-36-1
References:
1.  Gray, R.W., Omdahl, J.L., Ghazarian, J.G. and De Luca, H.F. 25-Hydroxycholecalciferol-1-hydroxylase. Subcellular location and properties. J. Biol. Chem. 247 (1972) 7528–7532. [PMID: 4404596]
2.  Sakaki, T., Sawada, N., Takeyama, K., Kato, S. and Inouye, K. Enzymatic properties of mouse 25-hydroxyvitamin D3 1 α-hydroxylase expressed in Escherichia coli. Eur. J. Biochem. 259 (1999) 731–738. [PMID: 10092858]
3.  Sawada, N., Sakaki, T., Kitanaka, S., Takeyama, K., Kato, S. and Inouye, K. Enzymatic properties of human 25-hydroxyvitamin D3 1α-hydroxylase coexpression with adrenodoxin and NADPH-adrenodoxin reductase in Escherichia coli. Eur. J. Biochem. 265 (1999) 950–956. [PMID: 10518789]
[EC 1.14.15.18 created 1976 as EC 1.14.13.13, transferred 2016 to EC 1.14.15.18]
 
 


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