ExplorEnz: EC 1.14.14.95

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1.14.14.95

Accepted name:

germacrene A hydroxylase

Reaction:

(+)-germacrene A + 3 [reduced NADPH—hemoprotein reductase] + 3 O₂ = germacra-1(10),4,11(13)-trien-12-oate + 3 [oxidized NADPH—hemoprotein reductase] + 4 H₂O (overall reaction)
(1a) (+)-germacrene A + O₂ + [reduced NADPH—hemoprotein reductase] = germacra-1(10),4,11(13)-trien-12-ol + [oxidized NADPH—hemoprotein reductase] + H₂O
(1b) germacra-1(10),4,11(13)-trien-12-ol + O₂ + [reduced NADPH—hemoprotein reductase] = germacra-1(10),4,11(13)-trien-12-al + [oxidized NADPH—hemoprotein reductase] + 2 H₂O
(1c) germacra-1(10),4,11(13)-trien-12-al + O₂ + [reduced NADPH—hemoprotein reductase] = germacra-1(10),4,11(13)-trien-12-oate + [oxidized NADPH—hemoprotein reductase] + H₂O

For diagram of costunolide biosynthesis, click here

Other name(s):

GAO (gene name)

Systematic name:

(+)-germacrene-A,[reduced NADPH—hemoprotein reductase]:oxygen oxidoreductase (12-hydroxylating)

Comments:

A cytochrome P-450 (heme-thiolate) protein. This plant enzyme catalyses three steps in a pathway that leads to the biosynthesis of many sesquiterpenoid lactones.

Links to other databases:

BRENDA, EXPASY, KEGG, MetaCyc

References:

1.	Nguyen, D.T., Gopfert, J.C., Ikezawa, N., Macnevin, G., Kathiresan, M., Conrad, J., Spring, O. and Ro, D.K. Biochemical conservation and evolution of germacrene A oxidase in asteraceae. J. Biol. Chem. 285 (2010) 16588–16598. [DOI] [PMID: 20351109]
2.	Liu, Q., Manzano, D., Tanic, N., Pesic, M., Bankovic, J., Pateraki, I., Ricard, L., Ferrer, A., de Vos, R., van de Krol, S. and Bouwmeester, H. Elucidation and in planta reconstitution of the parthenolide biosynthetic pathway. Metab. Eng. 23 (2014) 145–153. [PMID: 24704560]

[EC 1.14.14.95 created 2011 as EC 1.14.13.123, transferred 2018 to EC 1.14.14.95]