The Enzyme Database

Your query returned 1 entry.    printer_iconPrintable version

EC 1.14.13.84     
Accepted name: 4-hydroxyacetophenone monooxygenase
Reaction: (4-hydroxyphenyl)ethan-1-one + NADPH + H+ + O2 = 4-hydroxyphenyl acetate + NADP+ + H2O
For diagram of reaction, click here
Other name(s): HAPMO
Systematic name: (4-hydroxyphenyl)ethan-1-one,NADPH:oxygen oxidoreductase (ester-forming)
Comments: Contains FAD. The enzyme from Pseudomonas fluorescens ACB catalyses the conversion of a wide range of acetophenone derivatives. Highest activity occurs with compounds bearing an electron-donating substituent at the para position of the aromatic ring [1]. In the absence of substrate, the enzyme can act as an NAD(P)H oxidase (EC 1.6.3.1).
Links to other databases: BRENDA, EXPASY, KEGG, MetaCyc, CAS registry number: 156621-13-5
References:
1.  Kamerbeek, N.M., Moonen, M.J., van der Ven, J.G., van Berkel, W.J.H., Fraaije, M.W. and Janssen, D.B. 4-Hydroxyacetophenone monooxygenase from Pseudomonas fluorescens ACB: a novel flavoprotein catalyzing Baeyer-Villiger oxidation of aromatic compounds. Eur. J. Biochem. 268 (2001) 2547–2557. [PMID: 11322873]
2.  Kamerbeek, N.M, Olsthoorn, A.J.J., Fraaije, M.W. and Janssen, D.B. Substrate specificity of a novel Baeyer-Villiger monooxygenase, 4-hydroxyacetophenone monooxygenase. Appl. Environ. Microbiol. 69 (2003) 419–426. [PMID: 12514023]
[EC 1.14.13.84 created 2004]
 
 


Data © 2001–2017 IUBMB
Web site © 2005–2017 Andrew McDonald