The Enzyme Database

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EC 1.14.13.78     
Accepted name: ent-kaurene oxidase
Reaction: ent-kaur-16-ene + 3 NADPH + 3 H+ + 3 O2 = ent-kaur-16-en-19-oate + 3 NADP+ + 4 H2O (overall reaction)
(1a) ent-kaur-16-ene + NADPH + H+ + O2 = ent-kaur-16-en-19-ol + NADP+ + H2O
(1b) ent-kaur-16-en-19-ol + NADPH + H+ + O2 = ent-kaur-16-en-19-al + NADP+ + 2 H2O
(1c) ent-kaur-16-en-19-al + NADPH + O2 = ent-kaur-16-en-19-oate + NADP+ + H2O
For diagram of gibberellin A12 biosynthesis, click here
Systematic name: ent-kaur-16-ene,NADPH:oxygen oxidoreductase (hydroxylating)
Comments: Requires cytochrome P-450. Catalyses three sucessive oxidations of the 4-methyl group of ent-kaurene giving kaurenoic acid.
Links to other databases: BRENDA, EXPASY, KEGG, MetaCyc, CAS registry number: 149565-67-3
References:
1.  Ashman, P.J., Mackenzie, A. and Bramley, P.M. Characterization of ent-kaurene oxidase activity from Gibberella fujikuroi. Biochim. Biophys. Acta 1036 (1990) 151–157. [PMID: 2223832]
2.  Archer, C., Ashman, P.J., Hedden, P., Bowyer, J.R. and Bramley, P.M. Purification of ent-kaurene oxidase from Gibberella fujikuroi and Cucurbita maxima. Biochem. Soc. Trans. 20 (1992) 218. [PMID: 1397591]
3.  Helliwell, C.A., Poole, A., Peacock, W.J. and Dennis, E.S. Arabidopsis ent-kaurene oxidase catalyzes three steps of gibberellin biosynthesis. Plant Physiol. 119 (1999) 507–510. [PMID: 9952446]
[EC 1.14.13.78 created 2002]
 
 


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