The Enzyme Database

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Accepted name: 4-hydroxyphenylacetaldehyde oxime monooxygenase
Reaction: (Z)-4-hydroxyphenylacetaldehyde oxime + NADPH + H+ + O2 = (S)-4-hydroxymandelonitrile + NADP+ + 2 H2O
For diagram of dhurrin biosynthesis, click here
Other name(s): 4-hydroxybenzeneacetaldehyde oxime monooxygenase; cytochrome P450II-dependent monooxygenase; NADPH-cytochrome P450 reductase (CYP71E1); CYP71E1; 4-hydroxyphenylacetaldehyde oxime,NADPH:oxygen oxidoreductase
Systematic name: (Z)-4-hydroxyphenylacetaldehyde oxime,NADPH:oxygen oxidoreductase
Comments: This enzyme is involved in the biosynthesis of the cyanogenic glucoside dhurrin in sorghum, along with EC, tyrosine N-monooxygenase and EC, cyanohydrin β-glucosyltransferase.
Links to other databases: BRENDA, EXPASY, KEGG, MetaCyc
1.  MacFarlane, I.J., Lees, E.M. and Conn, E.E. The in vitro biosynthesis of dhurrin, the cyanogenic glycoside of Sorghum bicolor. J. Biol. Chem. 250 (1975) 4708–4713. [PMID: 237909]
2.  Shimada, M. and Conn, E.E. The enzymatic conversion of p-hydroxyphenylacetaldoxime to p-hydroxymandelonitrile. Arch. Biochem. Biophys. 180 (1977) 199–207. [PMID: 193443]
3.  Busk, P.K. and Møller, B.L. Dhurrin synthesis in sorghum is regulated at the transcriptional level and induced by nitrogen fertilization in older plants. Plant Physiol. 129 (2002) 1222–1231. [PMID: 12114576]
4.  Kristensen, C., Morant, M., Olsen, C.E., Ekstrøm, C.T., Galbraith, D.W., Møller, B.L. and Bak, S. Metabolic engineering of dhurrin in transgenic Arabidopsis plants with marginal inadvertent effects on the metabolome and transcriptome. Proc. Natl. Acad. Sci. USA 102 (2005) 1779–1784. [PMID: 15665094]
[EC created 2000, modified 2005]

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