A flavoprotein (FAD). The enzyme from Corynebacterium cyclohexanicum is highly specific for 4-hydroxybenzoate, but uses NADH and NADPH at approximately equal rates (cf. EC 22.214.171.124 4-hydroxybenzoate 3-monooxygenase). It is less specific for NADPH than EC 126.96.36.199.
Fujii, T. and Kaneda, T. Purification and properties of NADH/NADPH-dependent p-hydroxybenzoate hydroxylase from Corynebacterium cyclohexanicum. Eur. J. Biochem.147 (1985) 97–104. [DOI] [PMID: 3971979]
Seibold, B., Matthes, M., Eppink, M.H., Lingens, F., Van Berkel, W.J. and Muller, R. 4-Hydroxybenzoate hydroxylase from Pseudomonas sp. CBS3. Purification, characterization, gene cloning, sequence analysis and assignment of structural features determining the coenzyme specificity. Eur. J. Biochem.239 (1996) 469–478. [DOI] [PMID: 8706756]