Accepted name: methane monooxygenase (soluble)
Reaction: methane + NAD(P)H + H+ + O2 = methanol + NAD(P)+ + H2O
Other name(s): methane hydroxylase
Systematic name: methane,NAD(P)H:oxygen oxidoreductase (hydroxylating)
Comments: The enzyme is soluble, in contrast to the particulate enzyme, EC Broad specificity; many alkanes can be hydroxylated, and alkenes are converted into the corresponding epoxides; CO is oxidized to CO2, ammonia is oxidized to hydroxylamine, and some aromatic compounds and cyclic alkanes can also be hydroxylated, but more slowly.
1.  Colby, J. Stirling, D.I. and Dalton, H. The soluble methane mono-oxygenase of Methylococcus capsulatus (Bath). Its ability to oxygenate n-alkanes, n-alkenes, ethers, and alicyclic, aromatic and heterocyclic compounds. Biochem. J. 165 (1977) 395–402. [PMID: 411486]
2.  Hyman, M.R. and Wood, P.M. Methane oxidation by Nitrosomonas europaea. Biochem. J. 212 (1983) 31–37. [PMID: 6870854]
3.  Stirling, D.I. and Dalton, H. Properties of the methane mono-oxygenase from extracts of Methylosinus trichosporium OB3b and evidence for its similarity to the enzyme from Methylococcus capsulatus (Bath). Eur. J. Biochem. 96 (1979) 205–212. [PMID: 572296]
4.  Tonge, G.M., Harrison, D.E.F. and Higgins, I.J. Purification and properties of the methane mono-oxygenase enzyme system from Methylosinus trichosporium OB3b. Biochem. J. 161 (1977) 333–344. [PMID: 15544]
[EC created 1984, modified 2011]

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