The Enzyme Database

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EC 1.14.13.201     
Accepted name: β-amyrin 28-monooxygenase
Reaction: β-amyrin + 3 O2 + 3 NADPH + 3 H+ = oleanolate + 3 NADP+ + 4 H2O (overall reaction)
(1a) β-amyrin + O2 + NADPH + H+ = erythrodiol + NADP+ + H2O
(1b) erythrodiol + O2 + NADPH + H+ = oleanolic aldehyde + NADP+ + 2 H2O
(1c) oleanolic aldehyde + O2 + NADPH + H+ = oleanolate + NADP+ + H2O
For diagram of β-amyrin, β-seco-amyrin, 11-oxo-β-amyrin and soysapogenol biosynthesis, click here
Other name(s): CYP716A52v2; CYP716A12; CYP16A75; β-amyrin 28-oxidase
Systematic name: β-amyrin,NADPH:oxygen oxidoreductase (28-hydroxylating)
Comments: The enzyme, found in plants, is involved in the biosynthesis of oleanane-type triterpenoids, such as ginsenoside Ro. The enzyme from Medicago truncatula (barrel medic) (CYP716A12) can also convert α-amyrin and lupeol to ursolic acid and betulinic acid, respectively. The enzyme from Maesa lanceolata (false assegai) (CYP16A75) does not catalyse the reaction to completion, resulting in accumulation of both intermediates.
Links to other databases: BRENDA, EXPASY, KEGG, MetaCyc
References:
1.  Fukushima, E.O., Seki, H., Ohyama, K., Ono, E., Umemoto, N., Mizutani, M., Saito, K. and Muranaka, T. CYP716A subfamily members are multifunctional oxidases in triterpenoid biosynthesis. Plant Cell Physiol 52 (2011) 2050–2061. [PMID: 22039103]
2.  Han, J.Y., Kim, M.J., Ban, Y.W., Hwang, H.S. and Choi, Y.E. The involvement of β-amyrin 28-oxidase (CYP716A52v2) in oleanane-type ginsenoside biosynthesis in Panax ginseng. Plant Cell Physiol 54 (2013) 2034–2046. [PMID: 24092881]
3.  Moses, T., Pollier, J., Faizal, A., Apers, S., Pieters, L., Thevelein, J.M., Geelen, D. and Goossens, A. Unraveling the triterpenoid saponin biosynthesis of the African shrub Maesa lanceolata. Mol. Plant 8 (2015) 122–135. [PMID: 25578277]
[EC 1.14.13.201 created 2015]
 
 


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