The Enzyme Database

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EC 1.14.13.123     
Accepted name: germacrene A hydroxylase
Reaction: (+)-germacrene A + NADPH + H+ + O2 = germacra-1(10),4,11(13)-trien-12-ol + NADP+ + H2O
Systematic name: (+)-germacrene-A,NADPH:oxygen oxidoreductase (12-hydroxylating)
Comments: A heme-thiolate protein (P-450). This is probably part of the biosynthesis of many sesquiterpenoid lactones. In Lactuca sativa EC 1.14.13.123 is a mutifunctional enzyme with EC 1.1.1.314, germacrene A alcohol dehydrogenase [2].
Links to other databases: BRENDA, EXPASY, KEGG, MetaCyc
References:
1.  de Kraker, J.W., Franssen, M.C., Dalm, M.C., de Groot, A. and Bouwmeester, H.J. Biosynthesis of germacrene A carboxylic acid in chicory roots. Demonstration of a cytochrome P450 (+)-germacrene A hydroxylase and NADP+-dependent sesquiterpenoid dehydrogenase(s) involved in sesquiterpene lactone biosynthesis. Plant Physiol. 125 (2001) 1930–1940. [PMID: 11299372]
2.  Nguyen, D.T., Gopfert, J.C., Ikezawa, N., Macnevin, G., Kathiresan, M., Conrad, J., Spring, O. and Ro, D.K. Biochemical conservation and evolution of germacrene A oxidase in asteraceae. J. Biol. Chem. 285 (2010) 16588–16598. [PMID: 20351109]
[EC 1.14.13.123 created 2011]
 
 


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