The enzyme acts on multiple 3α-hydroxysteroids. Re-specific with respect to NAD+ or NADP+ [cf. EC 188.8.131.52, 3α-hydroxysteroid 3-dehydrogenase (Si-specific)]. Enzymes whose stereo-specificity with respect to NAD+ or NADP+ is not known are described by EC 184.108.40.2067, 3α-hydroxysteroid 3-dehydrogenase.
Björkhem, I. and Danielsson, H. Stereochemistry of hydrogen transfer from pyridine nucleotides catalyzed by Δ4-3-oxosteroid 5-β-reductase and 3-α-hydroxysteroid dehydrogenase from rat liver. Eur. J. Biochem.12 (1970) 80–84. [DOI] [PMID: 4392180]
Tomkins, G.M. A mammalian 3α-hydroxysteroid dehydrogenase. J. Biol. Chem.218 (1956) 437–447. [PMID: 13278351]